Part:BBa_K245046:Experience

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Applications of BBa_K245046

CcdB serves as positive selection marker since it is lethal to almost all BioBrick cell strains (E.coli DB3.1 strain is an exeption). While inserting BioBrick parts in plasmid, there exist a possibility of contamination of ligation mixture with uncut vector. If vector in which one plans to insert BioBrick part contains ccdB domain in multiple cloning site , cells that were transformed with unmodified vector express ccdB protein which results in cell death. For this reason any vector containing ccdB could only be propagated in resistant cell strains like DB3.1.

User Reviews

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